Angiogenesis of the blood‐brain barrier in vitro and the function of cerebral pericytes

  title={Angiogenesis of the blood‐brain barrier in vitro and the function of cerebral pericytes},
  author={Markus Ramsauer and Dorothee Krause and Rolf Dermietzel},
  journal={The FASEB Journal},
Cerebral pericytes constitute an essential component of the blood‐brain barrier (BBB) and are involved in blood vessel assembly. Recently, we reported on the induction of a BBB‐specific enzyme expressed by cerebral pericytes (pericytic aminopeptidase N/pAPN) in coculture with cerebral endothelial cells. We completed this in vitro BBB system by adding astrocytes to these mixed cultures of endothelial cells and pericytes. Under these triculture conditions, endothelial cells and pericytes… 

In Search of the Astrocytic Factor(s) Modulating Blood–Brain Barrier Functions in Brain Capillary Endothelial Cells In Vitro

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Differentiating embryonic neural progenitor cells induce blood–brain barrier properties

It is demonstrated that NPC–BMEC interactions are prevalent and for the first time demonstrates that NPC are capable of inducing BBB properties.

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This review article aimed to highlight the critical role of pericytes in restoration of the blood-brain barrier after injury by focusing on the dynamics ofpericytes and cross-talk with other cell types.

3D brain angiogenesis model to reconstitute functional human blood–brain barrier in vitro

A microfluidic model of CNS angiogenesis having three‐dimensional (3D) lumenized vasculature in concert with perivascular cells is presented, confirming the necessity of the angiogenic tri‐culture system (brain endothelium in direct interaction with pericytes and astrocytes) to attain essential phenotypes of BBB vasculatures.

Microvessel Remodeling in Cerebral Ischemia

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Human Vascular Pericytes and Cytomegalovirus Pathobiology

  • D. Alcendor
  • Biology, Medicine
    International journal of molecular sciences
  • 2019
Human pericytes have been shown to be targets for human cytomegalovirus (HCMV) infection and lytic replication that likely contribute to vascular inflammation and their potential role in subclinical vascular disease is discussed.



The Susceptibility of Cerebral Endothelial Cells to Astroglial Induction of Blood‐Brain Barrier Enzymes Depends on Their Proliferative State

It is concluded that direct contact ofBCECs with astroglial cells is necessary for the induction of the BBB phenotype in cultured BCECs and that this signal may be transferred from induced to noninduced BCEC's.

In vitro interaction of astrocytes and pericytes with capillary-like structures of brain microvessel endothelium.

An in vitro system is now available to study the interrelationships of these cell types and their interaction in development, regeneration and differentiation of the blood-brain barrier.

Blood–Brain Barrier Pericytes Are the Main Source of γ‐Glutamyltranspeptidase Activity in Brain Capillaries

The results emphasize the need for pure endothelial cells for the investigation of blood‐brain barrier characteristics and suggest that GGT is a specific marker for brain pericytes.

Astrocytes induce neural microvascular endothelial cells to form capillary‐like structures in vitro

This is the first demonstration of astroglial induction of angiogenesis in vitro and these findings support a functional role for perivascular astrocytes in the vascularization of neural tissue such as retina and brain.

Regulation of a Blood-Brain Barrier—Specific Enzyme Expressed by Cerebral Pericytes (Pericytic Aminopeptidase N/pAPN) under Cell Culture Conditions

It is shown that the aminopeptidase N of cerebral pericytes (pAPN) associated with the blood—brain barrier (BBB) is downregulated in pericytic cell cultures, and it is determined that the downregulation of pAPN occurs at the posttranscriptional level.

The effect of fibroblasts, vascular smooth muscle cells, and pericytes on sprout formation of endothelial cells in a fibrin gel angiogenesis system.

The results indicate that periendothelial cells contribute to angiogenesis not only by fibrinolysis and proteolytic permeation of the extracellular matrix, but also by the interactions of endothelial cells and pericyte-like cells, as frequently observed during neovessel formation in situ.

Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells

The results suggest that pericytes and SMCs can modulate EC growth by a mechanism that requires contact or proximity and postulate that similar interactions may operate to modulate vascular growth in vivo.

Role of the CNS microvascular pericyte in the blood‐brain barrier

Pericytes are a very important cellular constituent of the blood‐brain barrier and exhibit unique functional characteristics critical for the pathogenesis of a number of cerebrovascular, neurodegenerative, and neuroimmune diseases.

The Cerebral Microvessels in Culture, an Update

  • F. Joó
  • Biology
    Journal of neurochemistry
  • 1992
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