Anesthetics and Mild Hypothermia Similarly Prevent Hippocampal Neuron Death in an In Vitro Model of Cerebral Ischemia

@article{Popovi2000AnestheticsAM,
  title={Anesthetics and Mild Hypothermia Similarly Prevent Hippocampal Neuron Death in an In Vitro Model of Cerebral Ischemia},
  author={R Popovi{\'c} and R Liniger and Philip E. Bickler},
  journal={Anesthesiology},
  year={2000},
  volume={92},
  pages={1343-1349}
}
Background General anesthetics reduce neuron loss following focal cerebral ischemia in rodents. The relative efficacy of this action among different anesthetics clinically used for neuroprotection is uncertain. In addition, it remains unclear how anesthetics compare to neuroprotection afforded by mild hypothermia. This study was performed to evaluate the comparative effects of isoflurane, sodium pentothal, and mild hypothermia in a hippocampal slice model of cerebral ischemia and to determine… Expand
Isoflurane Prevents Delayed Cell Death in an Organotypic Slice Culture Model of Cerebral Ischemia
TLDR
In an in vitro model of simulated ischemia, 1% isoflurane is of similar potency to 10 &mgr;m MK-801 in preventing delayed cell death and Modulation of glutamate excitotoxicity may contribute to the protective mechanism. Expand
Isoflurane preconditioning reduces purkinje cell death in an in vitro model of rat cerebellar ischemia
TLDR
The results suggest that isoflurane preconditioning is neuroprotective, and the mechanisms of this protection seem to involve modulation of glutamate transporter activity. Expand
The Dose-Dependent Effects of Isoflurane on Outcome from Severe Forebrain Ischemia in the Rat
TLDR
Dose-dependent effects of preischemic administration of isoflurane on histologic and behavioral outcome after severe forebrain ischemia were observed, and Isoflurane MAC values <1.5 provided superior overall outcome relative to larger isofLurane concentrations. Expand
Effects of Neuroprotective Cocktails on Hippocampal Neuron Death in an In Vitro Model of Cerebral Ischemia
TLDR
The authors conclude that in this model, the strategy of antagonizing NMDA receptors appears more protective than fructose-1,6-bisphosphate, 2-chloroadenosine or ascorbate. Expand
Postconditioning with Isoflurane Reduced Ischemia-induced Brain Injury in Rats
TLDR
The results suggest that isoflurane administrated after OGD or brain ischemia provides neuroprotection and Mitochondrial adenosine 5′-triphosphate–sensitive potassium channels may be involved in this protection. Expand
Hypothermia protects human neurons
TLDR
Hypothermia protects stem cell-derived human neurons against insults relevant to stroke over a clinically relevant time frame and suggests an interaction in which protection benefits from reduction in available glucose under some but not all circumstances. Expand
&ggr;-Aminobutyric Acid-A Receptors Contribute to Isoflurane Neuroprotection in Organotypic Hippocampal Cultures
TLDR
It is likely that neuroprotection produced by isoflurane is multifactorial and includes actions at both GABAA and glutamate receptors and possibly other mechanisms. Expand
Dose-Dependent Influence of Sevoflurane Anesthesia on Neuronal Survival and Cognitive Outcome After Transient Forebrain Ischemia in Sprague-Dawley Rats
TLDR
Experimental models of cerebral ischemia should account for neuroprotective effects of sevoflurane with increasing concentrations, and a low inspired concentration should be used and fluctuations in the depth of anesthesia should be limited to ensure minimal interference on neuronal survival. Expand
Isoflurane decreases AMPA-induced dark cell degeneration and edematous damage of Purkinje neurons in the rat cerebellar slices
TLDR
It is suggested that isoflurane time-dependently but not concentration- Dependently reduce AMPA-induced neurotoxicity and may be one mechanism for the isof lurane-induced neuroprotection demonstrated in previous studies. Expand
Post-hypoxic hypothermia is protective in human NT2-N neurons regardless of oxygen concentration during reoxygenation
TLDR
It is concluded that hypothermia protects isolated neurons after in vitro hypoxia, and that this protection is not affected by hyperoxic, normoxic or hypoxic reoxygenation. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 42 REFERENCES
Isoflurane Reduces Ischemia-induced Glutamate Release in Rats Subjected to Forebrain Ischemia
TLDR
Isoflurane, in comparison with a N sub 2 O-fentanyl-anesthetized state, significantly attenuates excitatory neurotransmitter release in the hippocampus, and this effect of isoflurane is comparable to that of mild hypothermia. Expand
The effect of isoflurane on biochemical changes during and electrophysiological recovery after anoxia in rat hippocampal slices.
TLDR
Isoflurane does not significantly improve recovery of CA 1 pyramidal cells during anoxia nor does it attenuate the anoxic changes in ATP, sodium, and potassium after 4 or 7 min of anoxIA. Expand
Relative Neuroprotective Effects of Dizocilpine and Isoflurane During Focal Cerebral Ischemia in the Rat
TLDR
The hypothesis that NMDA receptor activation is injurious during focal ischemia and that amelioration of focal ischemic brain damage by N-methyl-D-aspartate receptor antagonists persists under normothermic conditions is supported. Expand
Mild hypothermia—a revived countermeasure against ischemic neuronal damages
TLDR
In experiments on rodents, hypothermia in the postischemic period that is introduced up to several hours after reperfusion and is maintained for one day followed by a slow rewarming, significantly protects hippocampal neurons against damage. Expand
Sympathetic ganglionic blockade masks beneficial effect of isoflurane on histologic outcome from near-complete forebrain ischemia in the rat.
TLDR
Isoflurane attenuated the peripheral sympathetic response to ischemia and improved histologic outcome compared with fentanyl and nitrous oxide, and this outcome benefit was reversed by sympathetic ganglionic blockade. Expand
Effects of lsoflurane and Hypothermia on Glutamate Receptor-mediated Calcium Influx in Brain Slices
TLDR
Isoflurane reduces the severity of key pathophyslologic events in an in vitro model of simulated cerebral ischemia and delays cellular injury induced by simulated ischemies, and hypothermia does not reduce the Intrinsic activity of cortical glutamate receptors but delays calcium accumulation during simulated is chemia. Expand
Halothane Reduces Focal Ischemic Injury in the Rat When Brain Temperature Is Controlled
TLDR
When brain temperature was maintained normothermic throughout the focal ischemic insult, a neurologic and histologic protective effect for halothane anesthesia was observed. Expand
Differential Effects of Anesthetic Agents on Outcome from Near‐complete but Not Incomplete Global Ischemia in the Rat
TLDR
Outcome from near‐complete but not incomplete cerebral ischemia depended on the anesthetic agent administered during the ischemic insult, and Isoflurane was protective compared with fentanyl and ketamine. Expand
Small Differences in Intraischemic Brain Temperature Critically Determine the Extent of Ischemic Neuronal Injury
TLDR
Results demonstrate that rectal temperature unreliably reflects brain temperature during ischemia, and that despite severe depletion of brain energy metabolites at all temperatures, small increments of intraischemic brain temperature markedly accentuate histopathological changes following 3-day survival. Expand
Neuroprotection by both NMDA and non-NMDA receptor antagonists in in vitro ischemia
TLDR
Organotypic hippocampal slice cultures are sensitive to both NMDA and non-NMDA glutamate receptor blockade and thus represent a useful in vitro system for the study of ischaemic neurodegeneration paralleling results reported using in vivo models of isCHAemia. Expand
...
1
2
3
4
5
...