Anesthesia of fish with benzocaine does not interfere with comet assay results.

@article{Gontijo2003AnesthesiaOF,
  title={Anesthesia of fish with benzocaine does not interfere with comet assay results.},
  author={Alisson Marques de Miranda Cabral Gontijo and Rodrigo Egydio Barreto and Guenter Speit and Victor Alexis Valenzuela Reyes and Gilson Luiz Volpato and Daisy Maria F{\'a}vero Salvadori},
  journal={Mutation research},
  year={2003},
  volume={534 1-2},
  pages={165-72}
}
Fish blood erythrocytes are frequently used as sentinels in biomonitoring studies. Usually, fish blood is collected by painful cardiac or caudal vein punctures. Previous anesthesia could decrease animal suffering but it is not known at present whether anesthesia can cause confounding effects. Therefore, using the alkaline single cell gel (SCG)/comet assay with blood erythrocytes of the cichlid fish Nile tilapia, we tested for a possible modulation of induced DNA damage (methyl methanesulfonate… CONTINUE READING

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Therefore , using the alkaline single cell gel ( SCG)/comet assay with blood erythrocytes of the cichlid fish Nile tilapia , we tested for a possible modulation of induced DNA damage ( methyl methanesulfonate ; MMS ) by the anesthetic benzocaine administered by bath exposure ( 80mg / l for approximately 10min ) .
Furthermore , benzocaine ( 80 - 600mg / l ) was tested for its genotoxic potential on fish erythrocytes in vitro and for potential interactions with two known genotoxins ( MMS and hydrogen peroxide ) .
TilapiaNo subtypeCichlids
Therefore , using the alkaline single cell gel ( SCG)/comet assay with blood erythrocytes of the cichlid fish Nile tilapia , we tested for a possible modulation of induced DNA damage ( methyl methanesulfonate ; MMS ) by the anesthetic benzocaine administered by bath exposure ( 80mg / l for approximately 10min ) .
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