Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3‐hydroxypropionate by metabolically engineered Pyrococcus furiosus

@article{Lian2016AncillaryCO,
  title={Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3‐hydroxypropionate by metabolically engineered Pyrococcus furiosus},
  author={H. Lian and Benjamin M. Zeldes and G. L. Lipscomb and Aaron B Hawkins and Y. Han and A. Loder and Declan Nishiyama and M. Adams and R. Kelly},
  journal={Biotechnology and Bioengineering},
  year={2016},
  volume={113}
}
  • H. Lian, Benjamin M. Zeldes, +6 authors R. Kelly
  • Published 2016
  • Biology, Medicine
  • Biotechnology and Bioengineering
  • Acetyl‐Coenzyme A carboxylase (ACC), malonyl‐CoA reductase (MCR), and malonic semialdehyde reductase (MRS) convert HCO3− and acetyl‐CoA into 3‐hydroxypropionate (3HP) in the 3‐hydroxypropionate/4‐hydroxybutyrate carbon fixation cycle resident in the extremely thermoacidophilic archaeon Metallosphaera sedula. These three enzymes, when introduced into the hyperthermophilic archaeon Pyrococcus furiosus, enable production of 3HP from maltose and CO2. Sub‐optimal function of ACC was hypothesized to… CONTINUE READING
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