Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation

  title={Ancient DNA from marine sediments: Precautions and considerations for seafloor coring, sample handling and data generation},
  author={Linda Armbrecht and Marco J. L. Coolen and Franck Lejzerowicz and Simon C. George and Karita Negandhi and Yohey Suzuki and Jennifer M. Young and Nicole R. Foster and Leanne K. Armand and Alan Cooper and Martin Ostrowski and Amaranta Focardi and Michael Stat and John W. Moreau and Laura S. Weyrich},
  journal={Earth-Science Reviews},

An Outlook for the Acquisition of Marine Sedimentary Ancient DNA (sedaDNA) From North Atlantic Ocean Archive Material

Studies incorporating sedimentary ancient DNA (sedaDNA) analyses to investigate paleo‐environments have increased considerably over the last few years, and the possibility of utilizing archived

The Potential of Sedimentary Ancient DNA to Reconstruct Past Ocean Ecosystems

Sedimentary ancient DNA (sedaDNA) offers a novel approach to investigating past marine ecosystems—from the smallest bacteria to phytoplankton and their predators— over geological timescales.

Paleogenetic approaches in tsunami deposit studies

Ancient DNA Research in Maritime and Underwater Archaeology: Pitfalls, Promise, and Future Directions

The rapid progression of DNA technology allows for the application of recently developed techniques to an ever-growing body of archaeological and environmental material recovered from submerged

The Challenges of Reconstructing Tropical Biodiversity With Sedimentary Ancient DNA: A 2200-Year-Long Metagenomic Record From Bwindi Impenetrable Forest, Uganda

Sedimentary ancient DNA has been proposed as a key methodology for reconstructing biodiversity over time. Yet, despite the concentration of Earth’s biodiversity in the tropics, this method has rarely

An optimized method for the extraction of ancient eukaryote DNA from marine sediments

An optimized extraction protocol integrating these steps, with an optional post‐library LMW size‐selection step to retain DNA fragments of ≤500 base pairs is presented, which should improve quantitative paleo‐monitoring of eukaryotes from marine sediments, as well as other studies relying on the detection of highly fragmented and degraded eUKaryote DNA in sediments.

Lake Sedimentary DNA Research on Past Terrestrial and Aquatic Biodiversity: Overview and Recommendations

The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays,

From sea surface to seafloor: a benthic allochthonous eDNA survey for the abyssal ocean

Future deep-sea eDNA surveys should examine oceanographic influences on eDNA transport and residence times, consider habitat heterogeneity at a range of spatial scales in the abyss, and aim to process large amounts of material per sample in order to increase the sampling coverage in these diverse deep ocean communities.



Preservation potential of ancient plankton DNA in Pleistocene marine sediments

The late-Pleistocene sediments mainly contained eukaryotic DNA of marine fungi and from terrestrial plants, which could have been introduced via the river Nile at the time of deposition and preserved in pollen grains and suggests a preferential degradation of marine plankton DNA.

Ancient DNA chronology within sediment deposits: are paleobiological reconstructions possible and is DNA leaching a factor?

It is indicated that DNA from sediments can still offer a rich source of information on past environments, provided that the risk from vertical migration can be controlled for and that physical remains of organisms or their ejecta need to have been incorporated in the sediments for their DNA to be detected.

Using palaeoenvironmental DNA to reconstruct past environments: progress and prospects

Palaeoenvironmental DNA (PalEnDNA) is defined as ancient DNA (aDNA) originating from disseminated genetic material within palaeoenvironmental samples. Sources of PalEnDNA include marine and lake

Planktonic foraminifera-derived environmental DNA extracted from abyssal sediments preserves patterns of plankton macroecology

Re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera, to assess the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown.

Ancient DNA complements microfossil record in deep-sea subsurface sediments

The potential of aDNA to better document the evolution of past marine ecosystems and opens new horizons for the development of deep-sea palaeogenomics is revealed.

Ancient and modern environmental DNA

EDNA has revealed an ancient forest in Greenland, extended by several thousand years the survival dates for mainland woolly mammoth in Alaska, and pushed back the dates for spruce survival in Scandinavian ice-free refugia during the last glaciation.

Isolation, amplification, and identification of ancient copepod DNA from lake sediments

A new method for the isolation, amplification, and identification of copepod DNA from whole lake sediments formed in the early Holocene to the present is reported, which involves amplification of a short (~300 base pair) DNA sequence that varies betweenCopepod species.

Ancient DNA derived from alkenone‐biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea

[1] Holocene sea surface temperatures (SST) of the Black Sea have been reconstructed using sedimentary C37 unsaturated alkenones assumed to be derived from the coccolithophorid haptophyte Emiliania