Ancient DNA Analysis of the Japanese Sea Lion (Zalophus californianus japonicus Peters, 1866): Preliminary Results Using Mitochondrial Control-Region Sequences

  title={Ancient DNA Analysis of the Japanese Sea Lion (Zalophus californianus japonicus Peters, 1866): Preliminary Results Using Mitochondrial Control-Region Sequences},
  author={Fimihiro Sakahira and Michiko Niimi},
  booktitle={Zoological Science},
Abstract In this study, we successfully extracted ancient DNA from skeletal remains of the Japanese sea lion—a species that is practically extinct—from archaeological sites and determined a partial sequence of its mitochondrial DNA control region. A molecular phylogenetic tree constructed by the neighbor-joining (NJ) method showed that the sequences from Japanese sea lions clustered together, with a high bootstrap value, and that this cluster was closest to the California sea lion cluster. The… 

The First Molecular Evidence of Korean Zalophus japonicus (Otariidae: Sea Lions) from the Archaeological Site of Dokdo Island, Korea

The mitochondrial sequence was clearly distinct from two other sea lions, Z. californianus and Z. wollebaeki, and could provide useful information for species identification and genetic diversity of Z. japonicus.

The complete mitochondrial genome of Japanese sea lion, Zalophus japonicus (Carnivora: Otariidae) analyzed using the excavated skeletal remains from Ulleungdo, South Korea

Phylogenetic analysis showed that Z. japonicus was a sister species to Z. californianus with 98.61% nucleotide sequence identity among 11 pinniped species in the infraorder Pinnipedia, which supported the previous results.

Galápagos and Californian sea lions are separate species: Genetic analysis of the genus Zalophus and its implications for conservation management

A case is built for classifying the Galápagos sea lion, the Californian sea lion and the Japanese sea lion as true species using a set of different phylogenetic reconstruction approaches.

The First Population Simulation for the Zalophus japonicus (Otariidae: Sea Lions) on Dokdo, Korea

The Japanese sea lion (Z. japonicus) has been regarded as an extinct species since the last report on Dokdo in 1951. Not much ecological information on the Z. japonicus on Dokdo (hereafter Dokdo sea

Pinniped taxonomy: review of currently recognized species and subspecies, and evidence used for their description

Morphologic and genetic criteria used to recognize pinniped species and subspecies are evaluated individually for all taxa in the three families: Otariidae (sea lions and fur seals), Odobenidae (walruses) and Phocidae (seals).


This chapter discusses how to identify the different types of fruit trees based on their fruit-like properties and some of the strategies used to identify them.

Le marsouin commun et le phoque gris en mer d'Iroise et le long de la façade Atlantique française : génétique des populations et modifications de l'écosystème

Les mammiferes marins sont des especes cles des ecosystemes. Ils subissent un nombre important de modifications de leur milieu qui necessitent la mise en place de strategie de conservation. Pour cela



Ancient DNA Analysis of Brown Bear (Ursus arctos) Remains from the Archeological Site of Rebun Island, Hokkaido, Japan

It is demonstrated that juvenile ancient bears of Rebun Island were originated from southern Hokkaido, which was an outside area of the Okhotsk Culture and belonged to the Epi-Jomon Culture with a close relation to a northern part of the Tohoku district.

Fur seals and sea lions (Otariidae): Identification of species and taxonomic review

Recent re‐examination of the genetic basis of taxonomic diversity within otariids required matching by comprehensive new studies of skull morphometry based on large sample sizes, to provide a sound basis for re‐appraisal of species limits in the family.

Nucleotide Substitution Rate of Mammalian Mitochondrial Genomes

This study examines the evolutionary pattern of the different functional mtDNA regions as accurately as possible on the grounds of available data, revealing some important ``genomic laws.

Seals, Sea Lions, and Walruses: A Review of the Pinnipedia

The pinnipeds, as a group, are world-wide in distribution although they are limited essentially to continental shores, islands, and polar ice fields in the breeding season, therefore, their distribution is essentially one-dimensional, comparable to that of alcids among birds, certain fishes and many marine invertebrates.

Molecular Evolutionary Genetics

Recent developments of statistical methods in molecular phylogenetics are reviewed and it is shown that the mathematical foundations of these methods are not well established, but computer simulations and empirical data indicate that currently used methods produce reasonably good phylogenetic trees when a sufficiently large number of nucleotides or amino acids are used.

Mitochondrial DNA from Ancient Bones

The discovery that DNA can also be recovered from ancient hones has created new possibilities for the study of past populations, as bones are abundant archaeological remains and many museums throughout the world contain extensive and well-characterized osteological collections.

A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences

  • M. Kimura
  • Biology
    Journal of Molecular Evolution
  • 2005
Some examples were worked out using reported globin sequences to show that synonymous substitutions occur at much higher rates than amino acid-altering substitutions in evolution.


  • J. Felsenstein
  • Economics
    Evolution; international journal of organic evolution
  • 1985
The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.

Molecular Cloning: A Laboratory Manual

The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

The sensitivity of the commonly used progressive multiple sequence alignment method has been greatly improved and modifications are incorporated into a new program, CLUSTAL W, which is freely available.