Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

@article{Pbo1989AncientDE,
  title={Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.},
  author={Svante P{\"a}{\"a}bo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 6},
  pages={
          1939-43
        }
}
  • S. Pääbo
  • Published 1 March 1989
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
Several chemical and enzymatic properties were examined in the DNA extracted from dry remains of soft tissues that vary in age from 4 to 13,000 years and represent four species, including two extinct animals (the marsupial wolf and giant ground sloth). The DNA obtained was invariably of a low average molecular size and damaged by oxidative processes, which primarily manifest themselves as modifications of pyrimidines and sugar residues as well as baseless sites and intermolecular cross-links… 

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