Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

@article{Pbo1989AncientDE,
  title={Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.},
  author={Svante P{\"a}{\"a}bo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 6},
  pages={
          1939-43
        }
}
  • S. Pääbo
  • Published 1 March 1989
  • Biology
  • Proceedings of the National Academy of Sciences of the United States of America
Several chemical and enzymatic properties were examined in the DNA extracted from dry remains of soft tissues that vary in age from 4 to 13,000 years and represent four species, including two extinct animals (the marsupial wolf and giant ground sloth). The DNA obtained was invariably of a low average molecular size and damaged by oxidative processes, which primarily manifest themselves as modifications of pyrimidines and sugar residues as well as baseless sites and intermolecular cross-links… 

Figures and Tables from this paper

Degradation of ancient DNA

Ancient DNA: Prospects and limitations

The principal limitations of fossil DNA extraction and characterisation are discussed, including degradation and modification of ancient DNA, difficulties in amplification of target sequences via the polymerase chain reaction, and the special problems that contamination presents for amplification and verification of fossilDNA sequences.

Amplification and analysis of Miocene plant fossil DNA.

  • E. Golenberg
  • Geography
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 1991
It is indicated that analysis of Miocene fossil DNA can be replicated, and can, therefore, open up the prospects for future development of the field of molecular palaeontology.

Ancient DNA

The possibilities are considered of using ancient DNA in resolving issues of systematics and evolution of various animal taxa, population genetics of humans and rare species, taxonomic identification and paleontological reconstructions, geographic origin of populations, microbiological analysis of Paleontological and archeological finds, as well as some humanitarian aspects of its use.

Ancient DNA Studies in Physical Anthropology

▪ Abstract Nucleic acids are preserved in prehistoric samples under a wide range of depositional environments. The development of new molecular methods, especially the polymerase chain reaction, has

Molecular anthropology: Touching the past through ancient DNA retrieval. Methodological aspects

  • H. Witas
  • Biology
    Anthropological Review
  • 2001
The revolution which introduced new techniques of molecular biology applied to DNA analysis enormously accelerated the progress in most areas of medicine and biology. Techniques such as polymerase

Genetic analyses from ancient DNA.

The precautions and criteria necessary to ascertain to the greatest extent possible that results represent authentic ancient DNA sequences are discussed, which highlight some significant results and areas of promising future research.

Ancient DNA: Methods, progress, and perspectives

The evolving methods for manipulating and analyzing ancient DNA (aDNA) are reviewed here, as well as more recent applications of these methods to anthropologically relevant samples.

Chloroplast DNA sequence from a Miocene Magnolia species

The extraction of DNA from fossil leaf samples from the Miocene Clarkia deposit, the amplification of an 820-base pair DNA fragment from the chloroplast gene rbcL from a fossil of the genus Magnolia, and its subsequent sequencing extend the ability to analyse ancient DNA and may open new avenues into problems in palaeobotany, biogeography, and in the calibration of mutation rates.

Ancient DNA: Using molecular biology to explore the past

  • T. A. BrownK. A. Brown
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1994
Ancient DNA has been discovered in many types of preserved biological material, including bones, mummies, museum skins, insects in amber and plant fossils, and has become an important research tool
...

References

SHOWING 1-9 OF 9 REFERENCES

Effects of Ionizing Radiation

Proceedings of the International Conference on the Peaceful Uses of Atomic EnergyHeld in Geneva, 8 August–20 August, 1955. Vol. 7: Nuclear Chemistry and Effects of Irradiation. Pp. x + 691. 10

Catalogue ofEgyptian Antiquities in the British Museum: Mummies and Human Remains (British

  • Museum, London),
  • 1968

Molecular Cloning:A Laboratory Manual (Cold Spring Harbor Lab., Cold Spring Harbor, NY)

  • 1982

Mummies, Disease and Ancient Culture

  • 1980

Cold Spring Harbor Symp

  • Quant. Biol
  • 1986

Vestn

  • Akad . Med . Nauk SSSR
  • 1983

Munchener Mumien (Lipp GmbtH, Munchen

  • 1985

Munchener Mumien (Lipp GmbtH, Munchen, F.R.G.)

  • 1985