Ancient Ciphers: Translation in Archaea

  title={Ancient Ciphers: Translation in Archaea},
  author={P. Dennis},
Examination of the M. jannaschii genome sequence revealed the presence of eleven genes encoding proteins implicated in translation initiation, three implicated in elongation, and one implicated in termination-release (Bult et al. 1996xBult, C.J., White, O., Olsen, G.J., Zhou, L., Fleischmann, R.D., Sutton, G.G., Blake, J.A., FitzGerald, L.M., Clayton, R.A., Gocayne, J.D. et al. Science. 1996; 273: 1058–1072Crossref | PubMedSee all ReferencesBult et al. 1996). Surprisingly, of the 11 putative… Expand
The archaeal eIF2 homologue: functional properties of an ancient translation initiation factor
The functional characterization of the archaeal homologue, a/eIF2, is reported, which demonstrates that the trimeric factor reconstituted from the recombinant polypeptides has properties similar to those of its eukaryal homologue: it interacts with GTP and Met-tRNAi, and stimulates binding of the latter to the small ribosomal subunit. Expand
Functional Molecular Mapping of Archaeal Translation Initiation Factor 2*
This study characterize the role of each subunit in the binding of the methionylated initiator tRNA and suggests a possible contribution of aIF2α to formation of a productive complex between a IF2 and the small ribosomal subunit is envisaged. Expand
Identification in the Ancient Protist Giardia lamblia of Two Eukaryotic Translation Initiation Factor 4E Homologues with Distinctive Functions
The identification and characterization of two eIF4E homologues in an ancient protist, Giardia lamblia, are described, suggesting a novel function not yet observed among other eif4Es in eukaryotes. Expand
Two different mechanisms for ribosome/mRNA interaction in archaeal translation initiation
The data suggest that archaea such as Sulfolobus routinely use two distinct mechanisms for translational initiation, resembling the pathway prevalent in present‐day bacteria, whereas ‘leaderless’ initiation, reminiscent of the eukaryotic pathway, would operate on monocistronic mRNAs and on opening cistrons of polycistronicmRNAs. Expand
Recent Advances in Archaeal Translation Initiation
This review focuses on archaeal translation initiation highlighting its relationships with either the eukaryotic or the bacterial world and the use of leaderless and leaderless mRNAs is analyzed. Expand
The Archaeal Elongation Factor EF-2 Induces the Release of aIF6 From 50S Ribosomal Subunit
Molecular modeling, using published structural data of closely related homologous proteins, elucidated the mechanistic interplay between the aIF6, aSBDS, and aEF2 on the ribosome surface and suggested that a conformational rearrangement of a EF2, upon GTP hydrolysis, promotes aIF 6 ejection. Expand
The large subunit of initiation factor aIF2 is a close structural homologue of elongation factors
Examination of the nucleotide‐complexed aIF2γ structures suggests mechanisms of action and tRNA binding properties similar to those of an elongation factor, and implications for the mechanism of translation initiation in both eukarya and archaea are discussed. Expand
Functional diversity of the eukaryotic translation initiation factors belonging to eIF4 families
This review analyzes the latest advances concerning the functionality of members of the eIF4 families in eukaryotic organisms and discusses the implications of this in the context of the current understanding of regulation of the translation initiation process. Expand
Molecular Mechanism of Scanning and Start Codon Selection in Eukaryotes
  • A. Hinnebusch
  • Biology, Medicine
  • Microbiology and Molecular Reviews
  • 2011
A robust molecular model now exists, describing the roles of initiation factors, notably eukaryotic initiation factor 1 and eIF1A, in stabilizing an “open” conformation of the 40S subunit with Met-tRNAiMet bound in a low-affinity state conducive to scanning and in triggering rearrangement into a “closed’ conformation incompatible with scanning. Expand
Evolution of the eukaryotic translation termination system: origins of release factors.
It is suggested that the current translation termination system in eukaryotes evolved from the archaea-like version of eRF1, eRF3 was introduced into the system prior to the divergence of extant eukARYotes, including G. lamblia, which might be the first eUKaryotic branch among the organisms considered. Expand


Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.
A strongly conserved mRNA element was identified that is exclusively linked to selenoprotein genes that is located in the 3' untranslated region in six of the mRNAs and in the 5' untranlated region of the fdhA mRNA. Expand
Autogenous translational regulation of the ribosomal MvaL1 operon in the archaebacterium Methanococcus vannielii
It is suggested that a novel mechanism of translational feedback regulation exists in M. vannielii, a methanogenic archaebacterium, which encoded by the MvaL1 operon of Methanococcus vannelii with high ribosomal gene expression. Expand
A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties.
Analysis of the enzyme's ability to cleave tRNA(Trp) substrates lacking exon sequences demonstrated that the mature tRNA-like structure is not required in the substrate, and the possible relationship of this enzyme to other RNA endonucleases is discussed. Expand
Ribosomal RNA precursor processing by a eukaryotic U3 small nucleolar RNA-like molecule in an archaeon.
U3-mediated processing of pre-rRNA is not specific to eukaryotes; its origin predates the divergence of archaea and eukARYotes. Expand
tRNA-dependent asparagine formation