Ancestral Protein Reconstruction Yields Insights into Adaptive Evolution of Binding Specificity in Solute-Binding Proteins.

@article{Clifton2016AncestralPR,
  title={Ancestral Protein Reconstruction Yields Insights into Adaptive Evolution of Binding Specificity in Solute-Binding Proteins.},
  author={Ben Clifton and Colin J Jackson},
  journal={Cell chemical biology},
  year={2016},
  volume={23 2},
  pages={236-245}
}
The promiscuous functions of proteins are an important reservoir of functional novelty in protein evolution, but the molecular basis for binding promiscuity remains elusive. We used ancestral protein reconstruction to experimentally characterize evolutionary intermediates in the functional expansion of the polar amino acid-binding protein family, which has… CONTINUE READING