Anatomy of energetic changes accompanying urea-induced protein denaturation.

@article{Auton2007AnatomyOE,
  title={Anatomy of energetic changes accompanying urea-induced protein denaturation.},
  author={Matthew Auton and Luis Marcelo Fernandes Holthauzen and David Wayne Bolen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 39},
  pages={
          15317-22
        }
}
Because of its protein-denaturing ability, urea has played a pivotal role in the experimental and conceptual understanding of protein folding and unfolding. The measure of urea's ability to force a protein to unfold is given by the m value, an experimental quantity giving the free energy change for unfolding per molar urea. With the aid of Tanford's transfer model [Tanford C (1964) J Am Chem Soc 86:2050-2059], we use newly obtained group transfer free energies (GTFEs) of protein side-chain and… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 15 REFERENCES

Protein Sci

LM Holthauzen, DW Bolen
  • 2007

Protein Sci 16:293–298

LM Holthauzen, DW Bolen
  • 2007

Protein Sci 12:1522–1529

CC Mello, D Barrick
  • 2003

Methods in Molecular Biology, ed Murphy KP (Humana

DW Bolen
  • Totowa, NJ),
  • 2001
VIEW 1 EXCERPT

Protein Sci 10:2485–2497

ES Courtenay, MW Capp, MT Record, Jr.
  • 2001
VIEW 1 EXCERPT

Proteins Suppl 4:72–85

ES Courtenay, MW Capp, RM Saecker, MT Record, Jr.
  • 2000
VIEW 1 EXCERPT

Protein Sci

IV Baskakov, DW Bolen
  • 1999

Protein Sci 8:1314–1319

IV Baskakov, DW Bolen
  • 1999

Structure (London) 7:477–488

A Caflisch, M Karplus
  • 1999

Protein Sci 4:2138–2148

JK Myers, CN Pace, JM Scholtz
  • 1995
VIEW 1 EXCERPT