Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?

@inproceedings{Nakanishi2016AnatomyOR,
  title={Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?},
  author={Kotaro Nakanishi},
  booktitle={Wiley interdisciplinary reviews. RNA},
  year={2016}
}
RNA silencing is a eukaryote-specific phenomenon in which microRNAs and small interfering RNAs degrade messenger RNAs containing a complementary sequence. To this end, these small RNAs need to be loaded onto an Argonaute protein (AGO protein) to form the effector complex referred to as RNA-induced silencing complex (RISC). RISC assembly undergoes multiple and sequential steps with the aid of Hsc70/Hsp90 chaperone machinery. The molecular mechanisms for this assembly process remain unclear… CONTINUE READING