Anandamide uptake is consistent with rate-limited diffusion and is regulated by the degree of its hydrolysis by fatty acid amide hydrolase.

@article{Kaczocha2006AnandamideUI,
  title={Anandamide uptake is consistent with rate-limited diffusion and is regulated by the degree of its hydrolysis by fatty acid amide hydrolase.},
  author={Martin Kaczocha and Anita Hermann and Sherrye T. Glaser and Inge N. Bojesen and Dale G. Deutsch},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 14},
  pages={9066-75}
}
The uptake of arachidonoyl ethanolamide (anandamide, AEA) in rat basophilic leukemia cells (RBL-2H3) has been proposed to occur via a saturable transporter that is blocked by specific inhibitors. Measuring uptake at 25 s, when fatty acid amide hydrolase (FAAH) does not appreciably affect uptake, AEA accumulated via a nonsaturable mechanism at 37 degrees C. Interestingly, saturation was observed when uptake was plotted using unbound AEA at 37 degrees C. Such apparent saturation can be explained… CONTINUE READING