Analyzing proteome topology and function by automated multidimensional fluorescence microscopy

@article{Schubert2006AnalyzingPT,
  title={Analyzing proteome topology and function by automated multidimensional fluorescence microscopy},
  author={Walter Schubert and Bernd Bonnekoh and Ansgar J. Pommer and Lars Philipsen and Raik B{\"o}ckelmann and Yanina N. Malykh and Harald p. M. Gollnick and Manuela Friedenberger and Marcus Bode and Andreas W. M. Dress},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={1270-1278}
}
Temporal and spatial regulation of proteins contributes to function. We describe a multidimensional microscopic robot technology for high-throughput protein colocalization studies that runs cycles of fluorescence tagging, imaging and bleaching in situ. This technology combines three advances: a fluorescence technique capable of mapping hundreds of different proteins in one tissue section or cell sample; a method selecting the most prominent combinatorial molecular patterns by representing the… Expand
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