Analyzing endodontic infections by deep coverage pyrosequencing.

  title={Analyzing endodontic infections by deep coverage pyrosequencing.},
  author={Lina Li and William W L Hsiao and Renu Nandakumar and Sara M Barbuto and Emmanuel F. Mongodin and Bruce J Paster and Claire Fraser-Liggett and Ashraf Fouad},
  journal={Journal of dental research},
  volume={89 9},
Bacterial diversity in endodontic infections has not been sufficiently studied. The use of modern pyrosequencing technology should allow for more comprehensive analysis than traditional Sanger sequencing. This study investigated bacterial diversity in endodontic infections through taxonomic classification based on 16S rRNA gene sequences generated by 454 GS-FLX pyrosequencing and conventional Sanger capillary sequencing technologies. Sequencings were performed on 7 specimens from endodontic… CONTINUE READING