Analytical validation of the GeXP analyzer and design of a workflow for cancer-biomarker discovery using multiplexed gene-expression profiling

@article{Rai2009AnalyticalVO,
  title={Analytical validation of the GeXP analyzer and design of a workflow for cancer-biomarker discovery using multiplexed gene-expression profiling},
  author={Alex J. Rai and Rashmi M Kamath and William L. Gerald and Martin Fleisher},
  journal={Analytical and Bioanalytical Chemistry},
  year={2009},
  volume={393},
  pages={1505-1511}
}
The GeXP genetic analysis system allows for multiplexed detection and quantitation of up to 35 genes in 192 samples in a single analysis. The analytical procedure includes modified reverse transcription and PCR amplification, followed by capillary electrophoretic separation. RNA material from multiple sample types can be used, including blood, cell lines, and tissue material. This instrumentation has a lower limit of detection of <2 ng with a dynamic range greater than two orders of magnitude… 
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It is concluded that GeXP analysis is a robust, sensitive, and useful method when the transcripts of tens to few hundred genes are to be precisely quantified in a high number of samples.
Quantifying 35 transcripts in a single tube: Model-based calibration of the GeXP RT-PCR assay
TLDR
It is concluded that GeXP analysis is a robust, sensitive, and useful method when the transcripts of tens to few hundred genes are to be precisely quantified in a high number of samples.
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An accurate and precise method to observe gene expression in whole blood that might provide specific diagnostic information for coronary artery disease (CAD) and other related diseases was established and suggested that gene expression was related to CAD.
Genotyping of 11 human papillomaviruses by multiplex PCR with a GeXP analyzer
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The GeXP‐PCR assay had comparable sensitivity and specificity to those of reported multiple PCR assay and an increased detection of HPV 11 in samples with mixed infections and is a fast, sensitive, and high throughput method for the detection of multiple HPV infections.
Gene expression profile in breast cancer comprising predictive markers for metastatic risk.
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The multiplex gene expression profile indicated significantly downregulated levels of G protein-coupled receptor kinase interacting ArfGAP 2 and mitochondrial transcription termination factor genes in a lymph node-positive group of patients, with P values of 0.004 and 0.038, therefore, this in-house method using multiple genes of interest might be an alternative tool for prediction of breast cancer metastasis.
Simultaneous detection of eight avian influenza A virus subtypes by multiplex reverse transcription-PCR using a GeXP analyser
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The GeXP-multiplex RT-PCR assay is a rapid and relatively high-throughput method for detecting and identifying eight AIV subtypes that may infect humans.
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MAQC papers over the cracks
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