Analytical ultracentrifugation sedimentation velocity for the characterization of detergent-solubilized membrane proteins Ca++-ATPase and ExbB.

Abstract

We have investigated the potential of new methods of analysis of sedimentation velocity (SV) analytical ultracentrifugation (AUC) for the characterization of detergent-solubilized membrane proteins. We analyze the membrane proteins Ca(++)-ATPase and ExbB solubilized with DDM (dodecyl-beta-D: -maltoside). SV is extremely well suited for characterizing sample… (More)
DOI: 10.1007/s10867-008-9058-3

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@article{Salvay2007AnalyticalUS, title={Analytical ultracentrifugation sedimentation velocity for the characterization of detergent-solubilized membrane proteins Ca++-ATPase and ExbB.}, author={Andr{\'e}s Gerardo Salvay and Monica Santamaria and Marc le Maire and Christine Ebel}, journal={Journal of biological physics}, year={2007}, volume={33 5-6}, pages={399-419} }