Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.

  title={Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.},
  author={James L. Cole and Jeffrey W. Lary and Thomas P Moody and Thomas M. Laue},
  journal={Methods in cell biology},
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permit precise and selective observation of sedimentation in real time. In particular, the fluorescence… CONTINUE READING
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