Analytical sensitivity of three real-time PCR assays for measuring subtype B HIV-1 RNA.

@article{Saun2013AnalyticalSO,
  title={Analytical sensitivity of three real-time PCR assays for measuring subtype B HIV-1 RNA.},
  author={Karine Saun{\'e} and Constance Delaugerre and St{\'e}phanie Raymond and Florence Nicot and J{\'e}r{\^o}me Boineau and Christophe Pasquier and Jacques Izopet},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2013},
  volume={57 1},
  pages={80-3}
}
BACKGROUND Lack of HIV RNA during antiretroviral therapy (ART) is regarded as a desirable outcome. Commercial assays of HIV virus load now need to detect virus RNA concentrations below 50 c/ml and several of them have claimed a limit of detection (LOD) of 20-45 c/ml. OBJECTIVES AND STUDY DESIGN We have compared the performances of three commercial assays of HIV RNA, the Abbott RealTime HIV-1, the Qiagen Artus RG HIV-1 and the Roche Cobas Ampliprep Cobas TaqMan (CAPCTM) HIV-1 vs 2.0 using… CONTINUE READING