Analytical methodologies for the enantiodetermination of citalopram and its metabolites.

  title={Analytical methodologies for the enantiodetermination of citalopram and its metabolites.},
  author={Monica Budău and Gabriel Hancu and Aura Rusu and Daniela-Lucia Muntean},
Citalopram (CIT) is a highly selective serotonin reuptake inhibitor (SSRI) frequently used in the treatment of major depressive disorders. It has a chiral centre in its structure and is used in therapy both as a racemic mixture (R,S-CIT) and a pure enantiomer (S-CIT). The differences between the pharmacokinetic and pharmacological profiles of the two enantiomers are well established. Consequently, the development of new efficient chiral analysis methods for their enantiomeric separation is a… 
4 Citations
Interaction of citalopram and escitalopram with calf Thymus DNA: A spectrofluorometric, voltammetric, and liquid chromatographic approach.
The results of this study may enhance the understanding of the interaction between CIT or ESC and ct-dsDNA and can be considered as the pioneer for future studies to uncover possible hidden phenotypes of these compounds.
Enantioselectivity in Drug Pharmacokinetics and Toxicity: Pharmacological Relevance and Analytical Methods
The data gathered in this review intend to reinforce the importance of the enantioselectivity in pharmacokinetic processes and reunite innovative enantiOSElective analytical methods applied in Pharmacokinetic studies.
Recent sample pretreatment methods for determination of selective serotonin reuptake inhibitors (SSRIs) in biological samples.
The present paper discussed the recent development of sample preparation methods for SSRIs analysis and prospected the challenges and promising directions for the future of sample pretreatment methods in SSRI analysis.
Colorimetric hand-held sensors and biosensors with a small digital camera as signal recorder, a review
  • M. Pohanka
  • Materials Science
    Reviews in Analytical Chemistry
  • 2020
This review summarizes research on hand-held assays where small cameras like the ones integrated into smartphones are used and their practical applicability and relevant specifications are written here.


Stereoselective HPLC-assay for citalopram and its metabolites.
The present assay allows a rapid, sensitive, and reliable stereoselective determination of CIT and its (active) metabolites which can be applied for assessing pharmacokinetic parameters and evaluating putative relationships to clinical (side) effects.
Analysis of Enantiomers of Citalopram and Its Demethylated Metabolites in Plasma of Depressive Patients Using Chiral Reverse‐Phase Liquid Chromatography
The results suggest the need for studies on the relationships between clinical response and plasma levels of CIT enantiomers, and the co-medications seemed to have little influence on enantiomer ratios.
Enantioselective analysis of citalopram and demethylcitalopram in human and rat plasma by chiral LC-MS/MS: application to pharmacokinetics.
The results showed a higher proportion of the (-)-(R)-CITA in human and rat plasma, with S/R AUC ratios for CITA and DCITA of 0.28 and 0.44, respectively.
Determination of the enantiomers of citalopram, its demethylated and propionic acid metabolites in human plasma by chiral HPLC.
A stereoselective HPLC assay has been developed to analyze the enantiomers of citalopram and of its three main metabolites in plasma after their separation on a Chiracel OD column and preliminary results suggest that the pharmacologically relevant (+)-(S)-isomers of CIT and DCIT could be preferentially and stereoselectedively metabolized to CIT-PROP.
Enantioselective analysis of citalopram and escitalopram in postmortem blood together with genotyping for CYP2D6 and CYP2C19.
In samples positive for racemic citalopram using the screening method for forensic autopsy cases, up to 20% would have been misinterpreted in the absence of an enantioselective method.
Enantioselective analysis of citalopram and demethylcitalopram in human whole blood by chiral LC-MS/MS and application in forensic cases.
  • S. Johansen
  • Chemistry, Medicine
    Drug testing and analysis
  • 2017
The method showed an acceptable precision and accuracy as the obtained coefficient of variation, and bias values were ≤16% for all enantiomers, and was successfully applied to authentic blood samples from forensic investigations demonstrating that escitalopram was less frequent than citalsopram among all forensic cases.
Enantiomeric separation of citalopram and its metabolites by capillary electrophoresis
A simple and fast capillary electrophoretic method has been developed for the enantioselective separation of citalopram and its main metabolites, namely N‐desmethylcitalopram and
Enantiomeric separation of citalopram analogues by HPLC using macrocyclic glycopeptide and cyclodextrin based chiral stationary phases
ABSTRACT The enantiomeric separation of a novel series of twenty-eight racemic mixtures of citalopram analogues was performed by high performance liquid chromatography (HPLC). Due to the
Analysis of the enantiomers of citalopram and its demethylated metabolites using chiral liquid chromatography.
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma, and is well suited for pharmacokinetic and drug interaction investigations.
Enantiomers’ potential in psychopharmacology—a critical analysis with special emphasis on the antidepressant escitalopram
This review highlights some of the potential advantages and disadvantages that using single enantiomers might offer and emphasis is given to the treatment of depression and how the potential of one pure enantiomer-escitalopram, the S-enantiomer of the selective serotonin reuptake inhibitor cITALopram-appears to be fulfilling its preclinical promise in the clinic.