Analytical characterization of plasma membrane-derived vesicles produced via osmotic and chemical vesiculation.

@article{Sarabipour2015AnalyticalCO,
  title={Analytical characterization of plasma membrane-derived vesicles produced via osmotic and chemical vesiculation.},
  author={Sarvenaz Sarabipour and Robin Barry Chan and Bowen Zhou and Gilbert di Paolo and Kalina Hristova},
  journal={Biochimica et biophysica acta},
  year={2015},
  volume={1848 7},
  pages={
          1591-8
        }
}
Plasma membrane-derived vesicles are being used in biophysical and biochemical research as a simple, yet native-like model of the cellular membrane. Here we report on the characterization of vesicles produced via two different vesiculation methods from CHO and A431 cell lines. The first method is a recently developed method which utilizes chloride salts to induce osmotic vesiculation. The second is a well established chemical vesiculation method which uses DTT and formaldehyde. We show that… CONTINUE READING

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