Analytical centrifugation: equilibrium approach.

@article{Laue2001AnalyticalCE,
  title={Analytical centrifugation: equilibrium approach.},
  author={Thomas M. Laue},
  journal={Current protocols in protein science},
  year={2001},
  volume={Chapter 20},
  pages={Unit20.3}
}
The specific interaction of biological molecules with one another is fundamental to the biochemistry of all living things. Equilibrium sedimentation is a classic method of biochemistry that provides first-principle thermodynamic information about the molar mass, association energy, association stoichiometry, and thermodynamic nonideality of molecules in solution. It is one of the most rigorous, powerful, and readily adapted methods for characterizing solution interactions. 

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