Analysis of tumour-specific alterations in native specimens by PCR.

@article{Bohm1997AnalysisOT,
  title={Analysis of tumour-specific alterations in native specimens by PCR.},
  author={Michael Bohm and Ilse Wieland},
  journal={International journal of oncology},
  year={1997},
  volume={10 1},
  pages={131-9}
}
Native tumours, in contrast to cell lines, are usually heterogeneous, consisting of tumour cells, stroma, infiltrating leukocytes, necrotic cells, and surrounding normal tissue. Therefore, when using non-linear amplification and detection methods such as the PCR, the verification of the DNA from the tumour cells is mandatory to avoid equivocal or false results. Here, current methods to isolate tumour cells from native tumours are reviewed. The methods are: i) a variety of microdissection… CONTINUE READING