Analysis of transcripts derived from sequences upstream of the bidirectional mouse thymidylate synthase promoter.

@article{Lee1998AnalysisOT,
  title={Analysis of transcripts derived from sequences upstream of the bidirectional mouse thymidylate synthase promoter.},
  author={Taohua Lee and Lee F. Johnson},
  journal={Experimental cell research},
  year={1998},
  volume={242 1},
  pages={
          222-7
        }
}
  • T. Lee, L. Johnson
  • Published 10 July 1998
  • Biology, Medicine
  • Experimental cell research
The promoter of the mouse thymidylate synthase (TS) gene lacks a TATAA box and an initiator element and is capable of directing transcriptional initiation with approximately equal strength and over broad initiation windows in both directions. The goal of the present study was to determine if the TS promoter directs the transcription of a second gene that is upstream of the TS gene by characterizing the transcripts that correspond to the upstream sequences. RNA blot analyses revealed the… Expand
Transcriptional control elements of the rat thymidylate synthase promoter: evolutionary conservation of regulatory features.
TLDR
The sequence elements that are important for the transcription and regulation of the rat thymidylate synthase (TS) gene were analyzed and site-directed mutagenesis revealed that four elements were especially important for promoter activity. Expand
Regulation of thymidylate synthase gene : effect of E2F and p53 /
Thymidylate synthase (TS) is an essential enzyme and thus an important target for cancer chemotherapeutic drugs. The proper S-phase regulation of the mouse TS gene requires the presence of the TSExpand
Bidirectional activity and orientation‐dependent specificity of the rat aldolase C promoter in transgenic mice
TLDR
It is shown that the 115 bp sequence is able to direct bidirectional transcription in vivo but, surprisingly, transcriptional activity in the opposite direction is no more brain specific. Expand

References

SHOWING 1-10 OF 17 REFERENCES
Bidirectional promoter of the mouse thymidylate synthase gene.
TLDR
It is shown that an essential region near the 5' end of the initiation window that is required for promoter activity stimulates transcription with approximately equal strength in both directions and raises the possibility that an upstream gene and the TS gene may be coordinately induced at the G1/S phase boundary by a common set of control elements. Expand
Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites.
TLDR
The structure of the gene for thymidylate synthase from a 5-fluoro-2'-deoxyuridine-resistant 3T6 cell line that overproduces thymidine synthase 50-fold by virtue of gene amplification is isolated and analysis revealed that all of the coding regions were contained in a 12-kilobase segment of DNA. Expand
Lack of an initiator element is responsible for multiple transcriptional initiation sites of the TATA-less mouse thymidylate synthase promoter.
TLDR
The results indicate that there are no additional promoter elements downstream of the G/C box in the initiation window of mouse thymidylate synthase and why transcription initiates across such a broad region is determined. Expand
Multiple protein-DNA interactions in the TATAA-less mouse thymidylate synthase promoter.
TLDR
The mouse thymidylate synthase (TS) promoter lacks a TATAA box and directs transcriptional initiation at multiple sites over a 60 nucleotide region, indicating that it is important for expression of the TS gene. Expand
Structural and functional analysis of the human thymidylate synthase gene.
TLDR
The complete nucleotide sequence of the human thymidylate synthase (TS) gene was determined and its sequence characteristics were compared with those of the mouse TS gene and other cell cycle-regulated genes. Expand
Construction and expression of mouse thymidylate synthase minigenes.
TLDR
To determine if the minigenes that lack introns were capable of being regulated in a cell cycle-dependent manner, thymidylate synthase gene expression was measured in hamster cells that were stably transfected with the largest minigene and synchronized by serum-stimulation. Expand
Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene.
TLDR
Computer homology searches have revealed sequence similarity of the divergent transcripts with bacterial genes involved in DNA mismatch repair, and the divergently transcribed gene Rep-1 is named. Expand
Bidirectional promoter activity of the 5′ flanking region of the mouse thymidine kinase gene
TLDR
The 5′ flanking region of the gene coding for cytoplasmic thymidine kinase in the mouse was tested for promoter activity using the chloramphenicol acetyltransferase gene as reporter and it was found that the region can be devided into two parts, one of which carries promoter activity in the direction of TK, whereas the 5′‐half has promoterActivity in the opossite direction. Expand
Splicing signals are required for S-phase regulation of the mouse thymidylate synthase gene
TLDR
It is shown that both the promoter of the mouse TS gene and TS introns are necessary (although neither is sufficient) for S-phase-specific regulation of TS mRNA content, and that the splicing reaction itself, rather than a control sequence within the intron, is important for growth-regulated expression of the TS gene. Expand
Molecular cloning of DNA sequences complementary to mouse thymidylate synthase messenger RNA.
TLDR
The isolation of recombinant cDNA clones containing sequences corresponding to mouse thymidylate synthase mRNA was described and RNA corresponding to this plasmid was isolated by hybridization and translated in vitro to yield a protein that coelectrophoresed with authentic thymidsodium synthase. Expand
...
1
2
...