Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation

@inproceedings{Roca2008AnalysisOT,
  title={Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation},
  author={Hernan Roca and Renny T. Franceschi},
  booktitle={Nucleic acids research},
  year={2008}
}
Chromatin immunoprecipitation (ChIP) is a widely used technique for quantifying protein-DNA interactions in living cells. This method commonly uses fixed (crosslinked) chromatin that is fragmented by sonication (X-ChIP). We developed a simple new ChIP procedure for the immunoprecipitation of sonicated chromatin isolated from osteoblasts in the absence of crosslinking (N-ChIP). The use of noncrosslinked chromatin allowed development of a new modification of the ChIP assay: the combination of N… CONTINUE READING

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