Analysis of transcription and estrogen insensitivity in the female mouse after targeted disruption of the estrogen receptor gene.

@article{Couse1995AnalysisOT,
  title={Analysis of transcription and estrogen insensitivity in the female mouse after targeted disruption of the estrogen receptor gene.},
  author={John F. Couse and Sylvia W. Curtis and Todd F Washburn and Jonathan Lindzey and T S Golding and Dennis B. Lubahn and Oliver Smithies and Kenneth S. Korach},
  journal={Molecular endocrinology},
  year={1995},
  volume={9 11},
  pages={1441-54}
}
We employed homologous recombination in mouse embryonic stem cells to disrupt the estrogen receptor (ER) gene. Subsequently generated mice that are homozygous for the gene disruption, termed ERKO, possess no demonstrable wild-type ER by Western blot analysis. However, the presence of residual high affinity binding, as detected by [3H]estradiol binding assays and sucrose gradients in uterine extracts from ERKO females prompted further investigation of transcription and translation products from… CONTINUE READING
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