Analysis of the transforming potential of the human H-ras gene by random mutagenesis.

@article{Fasano1984AnalysisOT,
  title={Analysis of the transforming potential of the human H-ras gene by random mutagenesis.},
  author={Ottavio Fasano and Teri Aldrich and Fuyuhiko Tamanoi and Elizabeth J Taparowsky and Marceline A. M. van Furth and Michael Wigler},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 13},
  pages={4008-12}
}
Some tumor cells contain mutant ras genes that are capable of transforming NIH 3T3 cells. Those genes that have been analyzed arise from the wild-type, non-transforming ras genes by mutations producing single amino acid substitutions at position 12 or 61 of the encoded protein. We have performed random bisulfite-induced mutagenesis on the cloned wild-type human H-ras gene to find if mutations at other positions can activate the transforming potential of that gene. Most mutations are not… CONTINUE READING

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