In this study, allele and genotype frequencies for the short tandem repeat (STR) loci D16S539, D7S820, and D13S317 were investigated in 138 unrelated individuals (124 for D13S317, 125 for D7S820, 138 for D16S539) living in Marmara region by PCR technique using an allelic ladder consisting of these loci as a standard size marker. It was evaluated that these STR loci could be valuable for forensic and paternity identification and the results were compared with other population studies. These loci were simultaneously amplified according to Gene Print Silver STR triplex kit (Promega Corp.). Amplified producs were separated on 6% denaturing polyacrylamide gel, visualised by silver staining. Seven alleles (8-14) were identified for D16S539, eight alleles (7-14) for D7S820, and seven alleles (8-14) for D13S317. No new alleles were found. The population investigated showed no deviation from Hardy-Weinberg equilibrium in the loci D16S539, D7S820 and D13S317. Population data showed a combined discrimination power of 0.9993 and a combined power of exclusion of 0.9523.