Analysis of the thermodynamic non-ideality of proteins by sedimentation equilibrium experiments.

@article{Behlke1999AnalysisOT,
  title={Analysis of the thermodynamic non-ideality of proteins by sedimentation equilibrium experiments.},
  author={Joachim Behlke and Otto Ristau},
  journal={Biophysical chemistry},
  year={1999},
  volume={76 1},
  pages={13-23}
}
This paper presents a modified method to determine experimentally the second virial coefficient of protein solutions by sedimentation equilibrium experiments. The improvement is based on the possibility of fitting simultaneously up to seven radial concentration distribution curves of solutions with different loading concentrations. The possibility of precise determination of the second virial coefficient allows estimation of the net charge and the excluded volume of a monomeric protein… CONTINUE READING

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