Analysis of the specificity of Panton-Valentine leucocidin and gamma-hemolysin F component binding.

@article{Meyer2009AnalysisOT,
  title={Analysis of the specificity of Panton-Valentine leucocidin and gamma-hemolysin F component binding.},
  author={Florent Meyer and Raymonde Girardot and Yves Pi{\'e}mont and Gilles Pr{\'e}vost and Didier A. Colin},
  journal={Infection and immunity},
  year={2009},
  volume={77 1},
  pages={266-73}
}
In this study, the binding of F components of the staphylococcal bicomponent leukotoxins Panton-Valentine leucocidin (LukF-PV) and gamma-hemolysin (HlgB) on polymorphonuclear neutrophils (PMNs), monocytes, and lymphocytes was determined using labeled mutants and flow cytometry. Leukotoxin activity was evaluated by measuring Ca(2+) entry or pore formation… CONTINUE READING