Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.

@article{Haucke1997AnalysisOT,
  title={Analysis of the sorting signals directing NADH-cytochrome b5 reductase to two locations within yeast mitochondria.},
  author={V Haucke and Carlos Oca{\~n}a and Angelika H{\"o}nlinger and Kostas Tokatlidis and Nikolaus Pfanner and Gottfried Schatz},
  journal={Molecular and cellular biology},
  year={1997},
  volume={17 7},
  pages={4024-32}
}
Mitochondrial NADH-cytochrome b5 reductase (Mcr1p) is encoded by a single nuclear gene and imported into two different submitochondrial compartments: the outer membrane and the intermembrane space. We now show that the amino-terminal 47 amino acids suffice to target the Mcr1 protein to both destinations. The first 12 residues of this sequence function as a weak matrix-targeting signal; the remaining residues are mostly hydrophobic and serve as an intramitochondrial sorting signal for the outer… CONTINUE READING