Analysis of the slow germination of multiple individual superdormant Bacillus subtilis spores using multifocus Raman microspectroscopy and differential interference contrast microscopy.

@article{Zhang2012AnalysisOT,
  title={Analysis of the slow germination of multiple individual superdormant Bacillus subtilis spores using multifocus Raman microspectroscopy and differential interference contrast microscopy.},
  author={Pengfei Zhang and Lan-ying Kong and Guiwen Wang and Michelle K. Scotland and Sonali Ghosh and Barbara Setlow and Peter Setlow and Y-q Li},
  journal={Journal of applied microbiology},
  year={2012},
  volume={112 3},
  pages={526-36}
}
AIM To analyse the dynamic germination of hundreds of individual superdormant (SD) Bacillus subtilis spores. METHODS AND RESULTS Germination of hundreds of individual SD B. subtilis spores with various germinants and under different conditions was followed by multifocus Raman microspectroscopy and differential interference contrast microscopy for 12h and with temporal resolutions of ≤30s. SD spores germinated poorly with the nutrient germinant used to isolate them and with alternate… CONTINUE READING

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