Analysis of the sequence-specific interactions between Cro repressor and operator DNA by systematic base substitution experiments.

@article{Takeda1989AnalysisOT,
  title={Analysis of the sequence-specific interactions between Cro repressor and operator DNA by systematic base substitution experiments.},
  author={Yoshinori Takeda and Akinori Sarai and V. Rivera},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 2},
  pages={439-43}
}
We measured quantitatively the binding affinities of purified Cro repressor to the chemically synthesized wild-type and mutant OR1 operators, consisting of all three possible base-pair substitutions and of thymine to uracil substitutions at each base-pair position of the 17-base-pair operator sequence. The sequence-specific interactions between Cro repressor and the operator DNA occur at the symmetrically disposed outer 7-base-pair positions of each half operator and at the central base-pair… CONTINUE READING

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