Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction.

@article{Aitken1993AnalysisOT,
  title={Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction.},
  author={Robert John Aitken and Donna W. Buckingham and Hua Fang},
  journal={Journal of andrology},
  year={1993},
  volume={14 2},
  pages={132-41}
}
Protocols for the use of A23187 in assessing the ability of human spermatozoa to acrosome-react and exhibit sperm-oocyte fusion have been developed and the results compared in two independent cohorts of infertile patients. Both bioassays were found to depend upon such factors as the dose and formulation of A23187, the duration of exposure, and the amount and type of protein used to supplement the medium. An optimal protocol for the hamster oocyte penetration test comprised a 3-hour exposure to… CONTINUE READING

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