Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe.

@article{Soto1998AnalysisOT,
  title={Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe.},
  author={T. C. Rioja Soto and Joseph Fernandez and Alberto Castro Dom{\'i}nguez and Jero Vicente-Soler and Jos{\'e} Cansado and Mariano Gacto},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1443 1-2},
  pages={
          225-9
        }
}
We have cloned and sequenced the ntp1+ gene that codes for neutral trehalase in the fission yeast Schizosaccharomyces pombe. The ntp1+ gene product (Ntp1p) showed a 45-55% identity with neutral trehalases from other yeasts at the amino acid sequence level. However, in clear contrast to other neutral yeast trehalases so far characterized (which show two cAMP phospho-sites), only one consensus site for cAMP-dependent protein phosphorylation was found in Ntp1p. Northern blot hybridization… CONTINUE READING
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