Analysis of the mitochondrial DNA genome in the peripheral blood leukocytes of HIV-infected patients with or without lipoatrophy.

@article{Mccomsey2002AnalysisOT,
  title={Analysis of the mitochondrial DNA genome in the peripheral blood leukocytes of HIV-infected patients with or without lipoatrophy.},
  author={Grace A. Mccomsey and Duan-Jun Tan and Michael M. Lederman and Elizabeth Wilson and L. J. C. Wong},
  journal={AIDS},
  year={2002},
  volume={16 4},
  pages={513-8}
}
OBJECTIVE To investigate the molecular mechanisms of nucleoside analogue reverse transcriptase inhibitor (NRTI)-associated mitochondrial dysfunction. METHODS Peripheral blood samples were collected from 10 healthy individuals, 10 HIV-infected, NRTI-treated patients with lipoatrophy, and four HIV-infected patients naive to all antiretrovirals. DNA was isolated from the leukocytes and the mitochondrial genome analyzed for DNA depletion, deletions and point mutations. RESULTS We were not able… CONTINUE READING