Analysis of the microbial community structure by monitoring an Hg methylation gene (hgcA) in paddy soils along an Hg gradient.

@article{Liu2014AnalysisOT,
  title={Analysis of the microbial community structure by monitoring an Hg methylation gene (hgcA) in paddy soils along an Hg gradient.},
  author={Yu-rong Liu and Ri-Qing Yu and Yuan-ming Zheng and Ji-Zheng He},
  journal={Applied and environmental microbiology},
  year={2014},
  volume={80 9},
  pages={2874-9}
}
Knowledge of the diversity of mercury (Hg)-methylating microbes in the environment is limited due to a lack of available molecular biomarkers. Here, we developed novel degenerate PCR primers for a key Hg-methylating gene (hgcA) and amplified successfully the targeted genes from 48 paddy soil samples along an Hg concentration gradient in the Wanshan Hg mining area of China. A significant positive correlation was observed between hgcA gene abundance and methylmercury (MeHg) concentrations… CONTINUE READING
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