Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.

@article{Friedhoff1996AnalysisOT,
  title={Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.},
  author={Peter Friedhoff and B Kolmes and Oleg A. Gimadutdinow and Wolfgang Wende and Kurt L Krause and Alfred Pingoud},
  journal={Nucleic acids research},
  year={1996},
  volume={24 14},
  pages={2632-9}
}
Based on crystal structure analysis of the Serratia nuclease and a sequence alignment of six related nucleases, conserved amino acid residues that are located in proximity to the previously identified catalytic site residue His89 were selected for a mutagenesis study. Five out of 12 amino acid residues analyzed turned out to be of particular importance for the catalytic activity of the enzyme: Arg57, Arg87, His89, Asn119 and Glu127. Their replacement by alanine, for example, resulted in mutant… CONTINUE READING
19 Citations
24 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 24 references

Protein Sci

  • Miller, M D Krause
  • Protein Sci
  • 1996

Annu. Rev. Biophys. Biomol. Struct

  • D B Wigley
  • Annu. Rev. Biophys. Biomol. Struct
  • 1995

Biochemistry

  • G Meiss, P Friedhoff, O Gimadutdinow, M Hahn, A Pingoud
  • Biochemistry
  • 1995

Diploma thesis, Justus-Liebig-Universität

  • B Kolmes
  • Diploma thesis, Justus-Liebig-Universität
  • 1995

Nature

  • C D Mol, C.-F Kuo, M M Thayer, R P Cunningham, J A Tainer
  • Nature
  • 1995

Nature Struct. Biol

  • Miller, M D Tanner, J Alpaugh, M Benedik, M J Krause
  • Nature Struct. Biol
  • 1994

Nucleic Acids Res

  • P Friedhoff, O Gimadutdinow, A Pingoud
  • Nucleic Acids Res
  • 1994

( NO TITLE )

  • M J Fraser, R L Low
  • 1993

Arch. Biochem. Biophys

  • L Y Chen, H C Ho, Y C Tsai, T H Liao
  • Arch. Biochem. Biophys
  • 1993

Eur. J. Biochem

  • A Heydenreich, G Koellner, +6 authors W Saenger
  • Eur. J. Biochem
  • 1993

Similar Papers

Loading similar papers…