Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.

@article{Gao2006AnalysisOT,
  title={Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.},
  author={Yan-kun Gao and Mei Jiang and Tao Yang and Jiang-Ye Chen},
  journal={Acta biochimica et biophysica Sinica},
  year={2006},
  volume={38 3},
  pages={164-70}
}
hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained. The interaction between PLZF and hPFTAIRE1 was confirmed by beta-galactosidase assay and Leu growth activity. PLZF encodes a transcription factor belonging to the POZ/BTB domain… CONTINUE READING