Analysis of the horse VH repertoire and comparison with the human IGHV germline genes, and sheep, cattle and pig VH sequences

@inproceedings{Almagro2006AnalysisOT,
  title={Analysis of the horse VH repertoire and comparison with the human IGHV germline genes, and sheep, cattle and pig VH sequences},
  author={Juan C Almagro and Laisel Martinez and Sylvia L. Smith and Alejandro Alag{\'o}n and Judith S. Rodr{\'i}guez Est{\'e}vez and Jorge Francisco Paniagua},
  year={2006}
}
Abstract We have constructed a chimeric antibody single-chain Fv (scFv) fragments phage-displayed library that combines an invariant human V L chain with the repertoire of V H domains amplified from a horse immunized against scorpion venom. To gain insight into the equine V H repertoire, the V H sequences of 46 unique clones randomly chosen from the library prior to antigenic selection were analyzed. Comparisons with previously reported equine V H sequences, as well as with the repertoire of… CONTINUE READING