Development of a transposon-based marker system for mutation breeding in sorghum (Sorghum bicolor L.).
In Korea, chrysanthemum is the second-most popular cutflower next to roses and can be divided into two main groups: spray and standard types according to their usage. The standard-type chrysanthemums are often used for condolence, and the important traits of this type include lack of branching and a long flowering period. This study was performed to compare the polymorphisms, genetic diversity, and genetic distances between the original variety and gamma-irradiated in vitro plants using amplified fragment length polymorphism (AFLP) markers. The in vitro explants derived from a standard-type flower, ‘Migok’, were gamma-irradiated at 0, 30, 50, 70, and 100 Gy, and the resulting genetic variations were more diverse in the in vitro populations than in the plants derived from conventional cuttings. We identified 83% (866 bands) of the polymorphisms using 12 primer combinations, and the highest polymorphism was detected using the M-CAT/E-ACC combination with a 30 Gy irradiation treatment. In addition, the genetic diversity and polymorphic information content value were the highest at 30 Gy irradiation of the in vitro population, whereas the genetic distance was the furthest between the 30 Gy-irradiated and other populations. Therefore, 30 Gy is the most effective dosage for inducing in vitro genetic variations in standard-type Migok.