Analysis of the gene encoding cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 and characterization of enzymatic properties.

@article{Kim1998AnalysisOT,
  title={Analysis of the gene encoding cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 and characterization of enzymatic properties.},
  author={Tae Jip Kim and Jun Ho Shin and Jin Hee Oh and M J Kim and S B Lee and S Ryu and K Kwon and Jung Wan Kim and Eun Ha Choi and John F. Robyt and Kwan Hwa Park},
  journal={Archives of biochemistry and biophysics},
  year={1998},
  volume={353 2},
  pages={221-7}
}
The gene encoding cyclomaltodextrinase (CDase) was cloned from alkalophilic Bacillus sp. I-5. The nucleotide sequence of the gene was determined and the physicochemical properties of the enzyme were investigated. The gene had an open reading frame of 559 amino acids with a predicted molecular weight of 64,884. The enzyme was purified to near homogeneity from Escherichia coli cells carrying a recombinant plasmid that contained the CDase gene. The enzyme hydrolyzed cyclomaltoheptaose (beta-CD) 13… CONTINUE READING