Analysis of the flagellin (hag) gene of alkalophilic Bacillus sp. C-125.

  title={Analysis of the flagellin (hag) gene of alkalophilic Bacillus sp. C-125.},
  author={Yasuharu Sakamoto and K J Sutherland and Jin Tamaoka and Toshiko Kobayashi and Toshiaki Kudo and Koki Horikoshi},
  journal={Journal of general microbiology},
  volume={138 10},
Motility of the alkalophilic Bacillus sp. C-125, a flagellate bacterium, was demonstrated to be Na(+)- and pH-dependent. Flagellin protein from this strain was purified to homogeneity and the N-terminal sequence determined. Using the hag gene of Bacillus subtilis as a probe, the hag gene of Bacillus sp. C-125 was identified and cloned into Escherichia coli. Sequencing of this hag gene revealed that it encodes a protein of 272 amino acids (M(r) 29,995). The predicted N terminal sequence of this… CONTINUE READING

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