Analysis of the expression of the Notch3 receptor protein in adult lung cancer

Abstract

Abnormalities in the Notch signaling system are considered to play a role in the tumorigenesis of bronchiogenic carcinoma. The present study aimed to investigate the expression of Notch3 in adult lung cancer patients and its role in the pathogenesis of primary bronchiogenic carcinoma. The expression of the Notch3 protein in lung squamous cell carcinoma, adenocarcinoma, small cell carcinoma and corresponding non-tumor tissues was detected by immunohistochemistry. To investigate the expression of Notch3 in adenocarcinoma tissues, Notch3 mRNA and protein expression were measured with reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis, respectively. It was demonstrated that Notch3 had a stronger positive degree of expression in lung squamous cell carcinoma and adenocarcinoma compared with the corresponding non-tumor tissue (P<0.01). The expression of Notch3 in small cell carcinoma tissue was lower compared with that of the corresponding non-tumor tissue (P<0.01). The expression of Notch3 in the lung adenocarcinoma group was the highest of the three lung carcinoma groups (P<0.01). RT-PCR revealed that the expression of Notch3 mRNA in the lung adenocarcinoma group was higher than that of the normal lung group, but there was no statistically significant difference (P=0.05). The expression of Notch1 protein in the lung adenocarcinoma group was significantly higher compared with the normal lung group (P<0.01), as shown by western blot analysis. Notch3 may be involved in the pathogenesis of bronchogenic carcinoma, in particular in the promotion of the lung cancer oncogene, and a difference in its expression may exist in the various pathological types. Introduction Primary bronchial lung cancer (referred to as lung cancer) is the most common primary pulmonary malignant tumor, despite progress in surgical techniques, chemotherapy and radiotherapy. The 5-year survival rate of patients with lung cancer remains very low. Jemal et al revealed that the 5-year survival rate of patients with lung cancer was only 16% (1). It is currently thought that abnormality of the multi-intracellular transmission pathway is associated with lung cancer, and there is increasing interest in the role of Notch signaling systems in tumorigenesis (2). Four notch genes encoding four types of Notch receptors, Notch1-4, have been identified in mammals. At least five types of ligand are capable of binding with these receptors and altering their function. Previously, studies concerned with the pathogenesis of lung cancer have identified a differential expression of Notch components in different types of lung cancer. It is hypothesized that Notch1 and 3 are associated with non-small cell lung cancer (NSCLC) (3,4). The majority of studies consider that Notch1 is involved in cancer promotion (5,6) and, compared with Notch1, there are a small number of studies regarding Notch3. Additionally, whether the effects of Notch1 and 3 are similar remains controversial. To further investigate the role of Notch signaling in lung adenocarcinoma, on the basis of Notch1 research, cases of surgical resection of small cell lung cancer (SCLC), lung squamous cell carcinoma and adenocarcinoma for the past 2 years in our hospital were reviewed. Immunohistochemistry and imaging analysis were used to detect the expression of Notch3 in lung cancer and negative tissue margins. Preliminary results indicated that the differences in Notch3 expression in lung adenocarcinoma were the most prominent and, accordingly, those specimens were collected from adult thoracic (lung) cancer surgerical resections. Specimens were later diagnosed as lung adenocarcinoma in post-operative pathological sections or normal lung tissue samples between January 2011 and May 2012. The expression of Notch3 in lung cancer tissues was detected by reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis to further explore the role of Notch3 in adult lung adenocarcinoma. Analysis of the expression of the Notch3 receptor protein in adult lung cancer MIN ZHOU1, WEI-YUN JIN1, ZHI-WEN FAN2 and RUI-CHAO HAN2 1Department of Respiratory Medicine, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500; 2Department of Respirtory Medicine, The Third People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, P.R. China Received August 23, 2012; Accepted November 11, 2012 DOI: 10.3892/ol.2012.1033 Correspondence to: Professor Min Zhou, Department of Respiratory Medicine, Jinshan Branch of the Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, No. 147 Jian Kang Road, Shanghai 201500, P.R. China E-mail: shdrzhoumin@hotmail.com

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@inproceedings{Zhou2013AnalysisOT, title={Analysis of the expression of the Notch3 receptor protein in adult lung cancer}, author={Min Zhou and W X Jin and Zhi-wen Fan and Rui-chao Han}, year={2013} }