Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae.

@article{Amerik2000AnalysisOT,
  title={Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae.},
  author={Alexander Y Amerik and S. J. Li and Mark Hochstrasser},
  journal={Biological chemistry},
  year={2000},
  volume={381 9-10},
  pages={981-92}
}
Attachment of proteins to ubiquitin is reversed by specialized proteases called deubiquitinating enzymes (Dubs), which are also essential for ubiquitin precursor processing. In the genome of Saccharomyces cerevisiae, 17 potential DUB genes can be discerned. We have now constructed strains deleted for each of these genes. Surprisingly, given the essential nature of the ubiquitin system, none of the mutants is lethal or strongly growth defective under standard conditions, although a number have… CONTINUE READING