Analysis of the complex transcription termination region of the Escherichia coli rrnB gene.

@article{Orosz1991AnalysisOT,
  title={Analysis of the complex transcription termination region of the Escherichia coli rrnB gene.},
  author={Andr{\'a}s Orosz and Imre Boros and P{\'a}l Venetianer},
  journal={European journal of biochemistry},
  year={1991},
  volume={201 3},
  pages={
          653-9
        }
}
The complex terminator region of the Escherichia coli rrnB gene was analyzed by subcloning the terminators T1 and T2 and the inverted repeats IR1 and IR2 individually, or in various combinations, in a normal or inverted orientation into a terminator probe vector. The in vivo terminating efficiency was assayed by measuring the galactokinase activity encoded by the downstream galK gene. Termination efficiencies of all fragments were compared in two constructs, differing in the presence or absence… CONTINUE READING

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