Analysis of the complete genomic sequence of a novel virus, areca palm necrotic spindle-spot virus, reveals the existence of a new genus in the family Potyviridae

  title={Analysis of the complete genomic sequence of a novel virus, areca palm necrotic spindle-spot virus, reveals the existence of a new genus in the family Potyviridae},
  author={Ke Yang and Minyuan Ran and Zengping Li and Meijiao Hu and Li Zheng and Wenbo Liu and Peng-fei Jin and Weiguo Miao and Peng Zhou and Wentao Shen and Hongguang Cui},
  journal={Archives of Virology},
A novel virus, tentatively named “areca palm necrotic spindle-spot virus” (ANSSV), was identified in Areca catechu L. in Hainan, China, and its complete genomic sequence was determined. Its positive-sense single-stranded RNA genome is comprised of 9,437 nucleotides (nt), excluding the poly (A) tail, and contains one large open reading frame encoding a polyprotein of 3,019 amino acids (aa). A Blastp search showed that the polyprotein of ANSSV shared a maximum of 31%-32% aa sequence identity… 
7 Citations
Biological and Molecular Characterization of Two Closely Related Arepaviruses and Their Antagonistic Interaction in Nicotiana benthamiana
This study investigated the pathological properties, functional compatibility of viral elements, and interspecies interactions in the model plant, Nicotiana benthamiana, and demonstrated that ANRSV confers effective exclusion of ANSSV both in coinfection and super-infection assays.
A Newly Identified Virus in the Family Potyviridae Encodes Two Leader Cysteine Proteases in Tandem That Evolved Contrasting RNA Silencing Suppression Functions
Evidence is presented showing self-cleavage activity of these two proteins and define their cis- Cleavage sites, which suggest that the two closely related leader proteases of ANSSV have evolved differential and essential functions to concertedly maintain viral viability.
Arachis virus Y, a new potyvirid from Brazilian forage peanut (Arachis pintoi)
The complete nucleotide sequence of a new member of the family Potyviridae, which we propose to name “Arachis virus Y” (ArVY), is reported from forage peanut plants (Arachis pintoi) exhibiting
Translation of Plant RNA Viruses
This review focuses on advances in cap-independent translation and translation recoding in plant viruses.
Assessment of the Spatial Distribution and Risk Associated with Fruit Rot Disease in Areca catechu L.
The identified FRD risk areas in this study, which showed higher disease probability rates exceeding the threshold level, need to be monitored with the utmost care to contain and reduce the further spread of the disease in Karnataka.


The complete genome sequence of a novel virus, bellflower veinal mottle virus, suggests the existence of a new genus within the family Potyviridae
Phylogenetic analysis and comparison of the encoded amino acid sequences with those of other viruses demonstrated that the identified virus shows minimal sequence similarity to known viruses and should therefore be considered a member of a new genus in the family Potyviridae.
Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae
Based on its low sequence similarity to known members of the family Potyviridae and phylogenetic analysis, RoYMV appears to be a distinct, previously undescribed, member of this family.
Complete genomic sequence of a novel macluravirus, alpinia oxyphylla mosaic virus (AloMV), identified in Alpinia oxyphylla
The data above suggest that AloMV represents an isolate of a putative new member within the genus Macluravirus, and is likely to be closely related to cardamom mosaic virus.
Complete genome sequence of a novel velarivirus infecting areca palm in China
The complete genome of a novel virus, provisionally named areca palm velarivirus 1 (APV1), was identified in areca palm exhibiting leaf yellowing symptoms in Hainan province, China. The genome of
An overlapping essential gene in the Potyviridae
This work reports the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the +2 reading-frame, which suggests that other short overlapping genes may remain hidden even in well studied virus genomes and demonstrates the utility of the software package MLOGD as a tool for identifying such genes.
Molecular criteria for genus and species discrimination within the family Potyviridae
A phylogenetic analysis of the optimised nucleotide (nt) alignment of the entire ORFs of each fully-sequenced species in the family Potyviridae provided strong support for several subgroups within the genus Potyvirus, suggesting that the continuum of variation that is theoretically available is constrained or disrupted by molecular barriers that must have some biological significance.
Transcriptional slippage in the positive-sense RNA virus family Potyviridae
It is demonstrated that transcriptional slippage, specific to the viral RNA polymerase, results in a population of transcripts with an additional “A” inserted within a highly conserved GAAAAAA sequence, thus enabling expression of P3N‐PIPO.
ICTV Virus Taxonomy Profile: Potyviridae
The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide.
Molecular biology of potyviruses.
RNA Polymerase Slippage as a Mechanism for the Production of Frameshift Gene Products in Plant Viruses of the Potyviridae Family
The mechanism of polymerase slippage results in the alteration of newly synthesized RNA in the case of viruses, and the mechanism has been described.