Analysis of the catalytic mechanism of juvenile hormone esterase by site-directed mutagenesis.

@article{Ward1992AnalysisOT,
  title={Analysis of the catalytic mechanism of juvenile hormone esterase by site-directed mutagenesis.},
  author={Vernon K Ward and Bryony C Bonning and Tien L. Huang and Takahiro Shiotsuki and V N Griffeth and Bruce D Hammock},
  journal={The International journal of biochemistry},
  year={1992},
  volume={24 12},
  pages={1933-41}
}
1. Juvenile hormone esterase (JHE) is a serine hydrolase selective for hydrolysis of the conjugated methyl esters of insect juvenile hormones. 2. We have investigated the mechanism of catalytic action of this enzyme by site-directed mutagenesis of the cloned enzyme and expression of the mutants in a baculovirus system. 3. A series of individual mutations of JHE were made to residues possibly involved in catalysis of juvenile hormones, and which are highly conserved in both esterases and lipases… CONTINUE READING

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