Analysis of the alfalfa root transcriptome in response to salinity stress.

@article{Postnikova2013AnalysisOT,
  title={Analysis of the alfalfa root transcriptome in response to salinity stress.},
  author={Olga A. Postnikova and Jonathan Shao and Lev G. Nemchinov},
  journal={Plant \& cell physiology},
  year={2013},
  volume={54 7},
  pages={
          1041-55
        }
}
Salinity is one of the major abiotic factors affecting alfalfa productivity. Identifying genes that control this complex trait will provide critical insights for alfalfa breeding programs. To date, no studies have been published on a deep sequencing-based profiling of the alfalfa transcriptome in response to salinity stress. Observations gathered through research on reference genomes may not always be applicable to alfalfa. In this work, Illumina RNA-sequencing was performed in two alfalfa… 
View on PubMed
academic.oup.com
107 Citations
Highly Influential Citations
6
Background Citations
33
Methods Citations
14
Results Citations
6

107 Citations

Citation Type

Citation Type

Transcriptome Analysis of Salt Tolerant Common Bean (Phaseolus vulgaris L.) under Saline Conditions
TLDR
The data generated in this study will help in understanding the fundamentals of salt tolerance in common bean and will provide resources for functional genomic studies.
Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
TLDR
The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity, and various candidate genes involved in salinity stress can be investigated further to improve salinity tolerance in alfalfa.
Transcriptome Analyses Reveal Candidate Genes Potentially Involved in Al Stress Response in Alfalfa
TLDR
This study aimed to understand how alfalfa responds to Al stress by identifying and analyzing Al-stress-responsive genes in al falfa roots at the whole-genome scale, and found that the DEGs involved in ribosome, protein biosynthesis, and process, the citrate cycle, membrane transport, and hormonal regulation were preferentially enriched and regulated.
Transcriptomic analysis of Casuarina equisetifolia L. in responses to cold stress
TLDR
Functional network analysis indicate that modifications in the expression of genes involved in signal regulation by transcription factors, metabolic pathways including synthesis of carbohydrate, secondary metabolites, and antioxidant enzymes might play vital roles in responses to cold stress in C. equisetifolia.
De Novo Transcriptional Analysis of Alfalfa in Response to Saline-Alkaline Stress
TLDR
Physiology assays showed an increased concentration of reactive oxygen species and malondialdehyde and a decreased content of chlorophyll, indicating that anti-oxidation and detoxification play an important role in response to saline-alkaline stress.
Full-length transcript sequencing and comparative transcriptomic analysis to evaluate the contribution of osmotic and ionic stress components towards salinity tolerance in the roots of cultivated alfalfa (Medicago sativa L.)
TLDR
The first full-length transcriptome database for alfalfa root tips under continuous NaCl and mannitol treatments provides evidence that the response to salinity stress in al falfa includes both osmotic and ionic components.
Transcriptional profiling reveals that a MYB transcription factor MsMYB4 contributes to the salinity stress response of alfalfa
TLDR
It is proposed that the MsMYB4 products is likely directly involved in alfalfa’s response to salinity stress, as it significantly increased the plants’ salinity tolerance in an abscisic acid-dependent manner.
Transcriptome analysis of genes and pathways associated with salt tolerance in alfalfa under non-uniform salt stress.
De Novo Transcriptome Characterization, Gene Expression Profiling and Ionic Responses of Nitraria sibirica Pall. under Salt Stress
TLDR
These findings have identified potential genes involved in salt tolerance and in the reference transcriptome and have revealed the salt tolerance mechanism in N. sibirica Pall.
Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms
TLDR
The results suggest that maintaining this specific physiological status and/or plant adaptation to salt stress most likely arises by inhibition of plant growth in ZM through plant hormone interactions, and increases the understanding of the genetic basis for salt tolerance.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 78 REFERENCES
Comparative transcriptomic analysis of salt adaptation in roots of contrasting Medicago truncatula genotypes.
TLDR
It is proposed that an increase in this bHLH TF expression may be linked to the adaptation of M. truncatula to saline soil environments, and improved significantly root growth under salt stress.
Screening of genes induced by salt stress from Alfalfa
TLDR
An alfalfa cDNA library induced by salt stress was constructed by suppression subtraction hybridization (SSH) technology and deduced protein sequences revealed that 51 ESTs had identity similar to proteins with known function, while 24 could not be annotated at all.
Multiple traits associated with salt tolerance in lucerne: revealing the underlying cellular mechanisms.
TLDR
The results suggest that different lucerne genotypes employ at least two different mechanisms for salt tolerance, and that both cultivars showed much less depolarisation of leaf membrane potential than the sensitive cultivars and had better K+ retention ability in both root and leaf tissues.
Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems
TLDR
The results demonstrate that RNA-Seq can be successfully used for gene identification, polymorphism detection and transcript profiling in alfalfa, a non-model, allogamous, autotetraploid species.
Massive parallel sequencing of mRNA in identification of unannotated salinity stress-inducible transcripts in rice (Oryza sativa L.)
BackgroundMicroarray technology is limited to monitoring the expression of previously annotated genes that have corresponding probes on the array. Computationally annotated genes have not fully been…
Nodulation under salt stress of alfalfa lines obtained after in vitro selection for osmotic tolerance
An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis
TLDR
It is indicated that MsERF11 has the potential to be used for improving crop’s salt tolerance in areas where salinity is a limiting factor for agricultural productivity.
An expression database for roots of the model legume Medicago truncatula under salt stress
TLDR
A database called MtED is reported that contains gene expression profiles of the roots of M. truncatula based on time-course salt stress experiments using the Affymetrix Medicago GeneChip and is intended to help in selecting gene markers to improve abiotic stress resistance in legumes.
Mechanisms of plant salt response: insights from proteomics.
TLDR
A salt-responsive protein database is described by an integrated analysis of proteomics-based studies that contains 2171 salt- responsive protein identities representing 561 unique proteins from 34 plant species.
Identification and Selection for Salt Tolerance in Alfalfa (Medicago sativa L.) Ecotypes via Physiological Traits
TLDR
Alfalfa cultivars assessed for salt tolerance at mature plant stage showed higher content of K than other ecotypes but had lower K/Na ratio, and two ecotypes ‘Malekan’ and ‘Ahar-Hourand’ were better.
...
1
2
3
4
5
...