Analysis of the Primary Sequence of Human Myelin Basic Protein Peptides 1–44 and 90–170 by Fast Atom Bombardment Mass Spectrometry

  title={Analysis of the Primary Sequence of Human Myelin Basic Protein Peptides 1–44 and 90–170 by Fast Atom Bombardment Mass Spectrometry},
  author={Hubert A. Scoble and John N. Whitaker and Klaus Biemann},
  journal={Journal of Neurochemistry},
Abstract: The originally described sequence of human myelin basic protein peptide 45–89 has recently been shown to contain two errors which have now been resolved. In the present study fast atom bombardment mass spectrometry was utilized to analyze the primary sequence of the other portions, peptides 1–44 and 90–170 of human myelin basic protein. The results obtained confirm the accuracy of the primary sequence published for both of these terminal peptides. 
p-Cresol sulfate is the dominant component of urinary myelin basic protein like material.
The unexpected immunochemical degeneracy permitting a cross-reaction between p-cresol sulfate and a peptide of an encephalitogenic myelin protein is postulated to be based on shared conformational features.
Myelin basic protein in cerebrospinal fluid and other body fluids
Urinary MBPLM, though different in its features from that in CSF, may provide a correlate, not with acute demyelination in MS as is the case for CSf, but with progression of disease.
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Fast atom bombardment mass spectrometry has been used to confirm and correct regions from the amino acid sequences of three large proteins, glutaminyl- and glycyl-tRNA synthetase from Escherichia
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