Analysis of the Primary Sequence of Human Myelin Basic Protein Peptides 1–44 and 90–170 by Fast Atom Bombardment Mass Spectrometry

@article{Scoble1986AnalysisOT,
  title={Analysis of the Primary Sequence of Human Myelin Basic Protein Peptides 1–44 and 90–170 by Fast Atom Bombardment Mass Spectrometry},
  author={Hubert A. Scoble and John N. Whitaker and Klaus Biemann},
  journal={Journal of Neurochemistry},
  year={1986},
  volume={47}
}
Abstract: The originally described sequence of human myelin basic protein peptide 45–89 has recently been shown to contain two errors which have now been resolved. In the present study fast atom bombardment mass spectrometry was utilized to analyze the primary sequence of the other portions, peptides 1–44 and 90–170 of human myelin basic protein. The results obtained confirm the accuracy of the primary sequence published for both of these terminal peptides. 
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References

SHOWING 1-10 OF 16 REFERENCES
Amino acid sequence of the encephalitogenic basic protein from human myelin.
  • P. Carnegie
  • Biology, Chemistry
    The Biochemical journal
  • 1971
TLDR
The basic protein from human brain was digested with trypsin and other enzymes and the sequence of the 170 amino acids was determined and the localization of the encephalitogenic determinants was described.
Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.
  • B. Gibson, K. Biemann
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
Fast atom bombardment mass spectrometry has been used to confirm and correct regions from the amino acid sequences of three large proteins, glutaminyl- and glycyl-tRNA synthetase from Escherichia
Indicators of Disease Activity in Multiple Sclerosis: Studies of Myelin Basic Protein‐like Materials a
  • J. Whitaker
  • Biology, Chemistry
    Annals of the New York Academy of Sciences
  • 1984
TLDR
The amount of BP-like material that enters CSF and cross-reacts with BP peptide 43-88 is related to the recognition of an epitope or epitopes in the C-terminal portion of this peptide.
Myelin basic protein.
  • E. Day
  • Biology, Medicine
    Contemporary topics in molecular immunology
  • 1981
Myelin basic protein (MBP) has engaged the attention of molecular and cellular immunologists during the past decade because of its implication in the induction of the autoimmune disease experimental
Analysis of autoimmune demyelination
  • 1984
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