Analysis of the P1 Gene Sequences And The 3′-Terminal Sequences and Secondary Structures of the Single-Stranded RNA Genome of Potato Virus V

@article{Oruetxebarria2001AnalysisOT,
  title={Analysis of the P1 Gene Sequences And The 3′-Terminal Sequences and Secondary Structures of the Single-Stranded RNA Genome of Potato Virus V},
  author={Igor Oruetxebarria and Jari P T Valkonen},
  journal={Virus Genes},
  year={2001},
  volume={22},
  pages={335-343}
}
The immunocapture reverse transcriptase PCR method (IC-RT-PCR) was used to selectively amplify specific genome sequences of an isolate of Potato virus V (PVV, genus Potyvirus) from a potato plant infected by multiple viruses in the field in Finland. The sequences of the 5′- and 3′-non-translated regions (NTR) and the P1- and coat protein (CP)-encoding sequences were determined because they are the most variable genomic regions in potyviruses. The sequences of the new Finnish PVV isolate… CONTINUE READING
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